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KMID : 1149220230310010011
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Korean Journal of Oriental Medical Prescription
2023 Volume.31 No. 1 p.11 ~ p.19
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Effects of Herbal medicines in Pacemaker Potential of Colonic Intestinal Interstitial cells of Cajal in mice
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Choi Na-Ri
Jeong Hae-Jeong
Choi Woo-Gyun
Kim Byung-Joo
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Abstract
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Objectives : The purpose of this study was to examine the effects of herbal medicines on pacemaker potentials of large intestinal interstitial Cells of Cajal (ICC) in mice.
Methods : We made the ICC culture in large intestine in mice and used the electrophysiological method to record pacemaker potentials. Also we used MTT assay to check cell viability and examined the ICC protein expression by western blot.
Results : 1.Glycyrrhiza uralensis Fischer (GF) (50-150 μg/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 58.95 μg/ml. Angelica gigas (AG) (50-200 μg/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 77.22 μg/ml. Poncirus fructus (PF) (10-100 μg/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 13.39 μg/ml. Citrus unshiu S. Marcov. (CU) (10-500 μg/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 139.80 μg/ml. Gardenia jasminoides J. Ellis (GJ) (100-500 μg/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 78.70 μg/ml. Coptis chinensis (CC) (100-1000 μg/ml) induced pacemaker depolarization and decreased frequency with concentration-dependent manners. EC50 is 138.10 μg/ml. Scutellaria baicalensis (SB) (10-100 μg/ml) had no effects on pacemaker potentials and decreased frequency with concentration-dependent manners. IC50 is 18.34 μg/ml. Atractylodes macrocephala koidzumi (AM) (10-100 μg/ml) induced pacemaker hyperpolarizations and decreased frequency with concentration-dependent manners. IC50 is 18.54 μg/ml.
2. PF, SB and AM had no effects on cell death in large ICC.
3. PF increased the ANO1 and c-kit protein expression and SB and AM increased the c-kit protein expression in large ICC.
Conclusions : These results suggest that PF, SB, and AM are likely to be the optimal combination of herbal medicines that can be used to treat diseases such as gastrointestinal motility disorders such as irritable bowel syndrome.
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KEYWORD
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RHerbal medicines, Poncirus fructus, Scutellaria baicalensis, Atractylodes macrocephala koidzumi, Irritable bowel syndrome, Interstitial Cells of Cajal
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